Xiaoqing, Wei and Changzhong, Liu and Li, Gao (2018) Optimization of culture conditions for Paecilomyces lilacinus (Thom) Samson M-14. African Journal of Microbiology Research, 12 (36). pp. 873-878. ISSN 1996-0808
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Abstract
Paecilomyces lilacinus is known as an effective parasite on nematodes which cause diseases to plants. P. lilacinus shows potential as a biocontrol agent against plant parasitic nematodes. The objective of this study is to optimize culture methods including nutritional requirements and environmental factors. The optimized culture conditions for biomass yields of P. lilacinus M-14 were spore suspension on basal medium (sucrose 19.00 g, soy peptone 4.06 g, K2HPO4 1.00 g, KCl 0.50 g, MgSO4 0.50 g, FeSO4 0.01 g and 17.00 g Bactor) for the first stage culture of 4 days under room condition for fungal growth, and then moved to another medium (maltose 5.00 g, soy peptone 2.50 g, ZnSO4·7H2O 0.25 gL-1, Na2MoO4·2H2O 0.005 gL-1, H3BO4 0.005 gL-1, CuSO4·5H2O 0.01 gL-1 and 17.00 g Bactor) for another 4 days culture. The environmental factors combination was water potential -1.2 MPa/pH 3/light 12 h/temperature 29°C for biomass yields, and for sporulation of P. lilacinus M-14 under the environmental conditions, it was water potential -1.2 MPa/pH 3/24 h light/29°C. It will provide valuable insight into culturing of the biocontrol fungus.
Item Type: | Article |
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Subjects: | Research Asian Plos > Biological Science |
Depositing User: | Unnamed user with email support@research.asianplos.com |
Date Deposited: | 29 Mar 2023 08:13 |
Last Modified: | 23 Oct 2024 04:14 |
URI: | http://abstract.stmdigitallibrary.com/id/eprint/385 |