Optimization of Real-time Reverse Transcriptase Polymerase Chain Reaction for Detection of Dengue Virus

Azizi, Kaunara A. and Ndaro, Arnold J. and Maro, Athanasia and Saro, Adonira and Kavishe, Reginald A. (2020) Optimization of Real-time Reverse Transcriptase Polymerase Chain Reaction for Detection of Dengue Virus. Journal of Advances in Microbiology, 20 (8). pp. 1-7. ISSN 2456-7116

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Abstract

Aims: This study was set to optimize conditions for real time reverse transcriptase polymerase chain reaction (RT-PCR) for detection of dengue virus by using rapid and simple nucleic acid extraction method.

Methodology: One step and two step real time RT-PCR were evaluated in different PCR thermocyclers. Extraction of viral RNA was done by using a simple boom method.

Results: The real time RT-PCR technique was successfully optimized using simple and rapid method for purification of nucleic acid, ‘boom method’. The technique works better when performed in a two-step procedure and can works well with all range of real time PCR machines. The optimized real time RT-PCR used in the present study is a valuable and reliable technique for routine diagnosis of dengue. Further investigation on the cost effectiveness in adopting this technique for routine screening and monitoring of the dengue infection should be done.

Item Type: Article
Subjects: Research Asian Plos > Biological Science
Depositing User: Unnamed user with email support@research.asianplos.com
Date Deposited: 28 Feb 2023 03:58
Last Modified: 15 Oct 2024 05:55
URI: http://abstract.stmdigitallibrary.com/id/eprint/269

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