Skendrović, Dino and Primožič, Mateja and Rezić, Tonči and Vrsalović Presečki, Ana (2024) Mesocellular Silica Foam as Immobilization Carrier for Production of Statin Precursors. International Journal of Molecular Sciences, 25 (4). p. 1971. ISSN 1422-0067
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Abstract
The employment of 2-deoxyribose-5-phosphate aldolase (DERA) stands as a prevalent biocatalytic route for synthesizing statin side chains. The main problem with this pathway is the low stability of the enzyme. In this study, mesocellular silica foam (MCF) with different pore sizes was used as a carrier for the covalent immobilization of DERA. Different functionalizing and activating agents were tested and kinetic modeling was subsequently performed. The use of succinic anhydride as an activating agent resulted in an enzyme hyperactivation of approx. 140%, and the stability almost doubled compared to that of the free enzyme. It was also shown that the pore size of MCF has a decisive influence on the stability of the DERA enzyme.
Item Type: | Article |
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Subjects: | Research Asian Plos > Multidisciplinary |
Depositing User: | Unnamed user with email support@research.asianplos.com |
Date Deposited: | 07 Feb 2024 06:41 |
Last Modified: | 17 Oct 2024 05:08 |
URI: | http://abstract.stmdigitallibrary.com/id/eprint/2446 |